The proteins were obtained from the tissue proboscis, individual segments of single individuals, the species of which had previously been accurately determined, and were separated by isoelectric focusing IEF in polyacrylamide gels.
The enzymes were identified by their specific catalytic activities, and the general proteins were visualized by silver staining. All the isozymes proved to be monomorphic within each of the various polychaete populations.
Forstwirt single a single exception, it was impossible to distinguish geographically separated populations of the same species, because the band patterns were completely consistent within the species. The individual enzymes varied in their suitability for species differentiation.
In contrast, each species could be identified by its general protein pattern, although some species differed from others in the position of a few bands only. Individuals of N. Only in the case of N.
This is a preview of subscription content, log in to check access. Access options Instant access to the full article PDF.
Subscription will auto renew annually.